Keller-kiliani test for glycosides pdf

The majority of the saccharides found in cardiac glycosides are highly specific. Test for carbohydrates are used to identify and measure the carbohydrates in a sample. To 2 ml solvent extract glacial acetic acid added, followed by one drop 5% fecl 3 and conc. Development of a blue or violet colour that faded out in1 to 2 hrs shows it presence of cardinoloids. Cardiac glycosides comprise a steroidal aglycone having an unsaturated. Standard methods were used for the identification of cardiac glycosides, phenolic compounds, flavonoids, anthraquinones, and alkaloids. Tests for alkaloids the alkaloids have been tested by using dragendroffs test and wagners test. Classification according to the linkage between aglycone and glycone. In 2 ml plant extract, glacial acetic acid, one drop of 5% fecl 3 and conc. A 5 mg of each extract was treated with 1 ml of glacial acetic acid and few drops of ferric chloride solution in a test. It is also sometimes called dixkeller reagent, after e.

Brown ring at interphase is characteristic of deoxysugars in cardenolides. Some members have an aldehyde group rather than methyl group at c19 29. Reddish brown color was appeared at junction of the two liquid layers and upper layer appears bluish green indicated the presence of glycosides. Keller kiliani test for deoxy sugars in cardiac glycosides. Bioline international official site site updated regularly. In vitro antimicrobial activity and phytochemical analysis of some indian medicinal plants jigna parekh, sumitra v.

Preliminary phytochemical screening, quantitative analysis of. Test for cardiac glycosides keller kiliani test five ml of each extracts was treated with 2 ml of glacial acetic acid containing one drop of ferric chloride solution. A solution of glycosides is treated with a small amount of kedde reagent mix equal volumes of a 2% solution of 3, 5 dinitrobenzoic acid in menthol and a 7. Preliminary phytochemical screening of some compounds. Phenols are defined as compounds that have at least one hydroxyl group attached to a benzene ring. What are the best methods used for identification of. These sugars are part of cardiac glycosides like the digitalis and strophanthus. The cohn defined the theory, its use to identify, the principal components of digitalis.

Pdf phytochemical analysis of biologically active constituents of. Structure of the sugar moiety the sugar moiety is generally linked to the aglycone through the hydroxyl group at c 3. Uv spectroscopy of ethanolic extract of cassia obtusifolia seed s. Preliminary phytochemical screening of selected medicinal. Preliminary phytochemical screening of some compounds from. Phytochemical analysis of traditional medicinal plants and.

Tests for glycosides, flavanoid, saponin, cardiac,anthraquenone, salkowaski test, shinoda test, keller kiliani, foam and fluorescence test. Kellers reagent is the find different kinds of alkaloids via reactions, which produced the products with the colors. Examples of naturally occurring plant phenols are catechol, guaiacol, hydroquinone, or phloroglucinol. In vivo antiinflammatory potential of leaf extracts of. Cardiac glycosides, cardenolides, and bufadienolides, bear a structure resemblance to the. To 2 ml extract, add glacial acetic acid, one drop 5% fecl 3 and conc. In vitro antimicrobial activity and phytochemical analysis.

Preliminary pharmacognostic and phytochemical studies on. Fifty 50 mg of each extract was dissolved in 2 ml chloroform. Ppt glycosides powerpoint presentation free to view. Concentrated ch 3cooh and concentrated h 2so 4 were added carefully along the wall of test tube. Phytochemical, nutraceutical and antioxidant studies of the aerial. This was underlayed with 1 ml of concentrated sulphuric acid. H 2 so 4 was added to form a layer and presence of colour at interphase was noted. Test for glycosides 12 the extract is tested for free sugars. Phenolic glycosides phgs are glycosides of phenols or compounds which have in their structure a c 6c 1 or c 6c 3 unit, one carboxyl group, and one or more hydroxyl group.

Kellers reagent can refer to either of two different mixtures of acids in metallurgy, kellers reagent is a mixture of nitric acid, hydrochloric acid, and hydrofluoric acid, used to etch aluminum alloys to reveal their grain boundaries and orientations. B either insoluble or less soluble in non polar organic solvents. Legals test to the hydrolysate, 1ml of pyridine and a few drops of sodium nitroprusside solution was added and then made alkaline with sodium hydroxide solution. Methanol extract was obtained and the extract reduced to dryness. Test for coumarins 3 ml of 10% naoh was added to 2 ml of aqueous extract. Bromine water test the test solution treated with bromine water yield a pale yellow colour which indicates the presence of glycosides. Some members have an aldehyde group rather than methyl group at. Reddish brown coloration at the junction of both layers and bluish green color at the upper layer indicated the presence of glycosides. Kellerkiliani test and concentrate h 2 so 4 test confirmed the presence of glycosides in the methanolic plant extract. Glycosides consist of a sugar residue covalently bound to a different structure called the aglycone. Tests for glycosides keller kiliani test was used for the analysis of glycosides.

After complete removal of sugar, the extract in hydrolysed with dilute mineral acid and then tested for the glycone and aglycone moieties. Phytochemical screening and anthelmintic and antifungal activities. Other substituent groups may be present, for example additional hydroxy groups at c1,11,12,16 and 19. The sugar residue is in its cyclic form and the point of attachment is the hydroxyl group of the hemiacetal function. Reddish brown appears at junction of the two liquid layers and upper layer appears bluish green indicates the presence of glycosides kokate et al. Thus kellerkiliani reaction in acetic acid, ferric chloride and sulfuric acid. After cooling 23 drops of ferric chloride was added. Development of a blue or violet colour that faded out in l to 2 hrs shows it. Phytochemical analysis and antimicrobial activity of. Phenols were detected by ferric chloride test 31, 32.

Following methods are good for the analysis of glycosides in herbal extracts. To that few drops of dilute h2so4 was added to form a layer. The appearance of reddish brown color at the junction of the two liquid layers and upper layer appears bluish green confirmed the positive for the presence of glycosides. If glycoside is present yellow to orange colour will be seen. Specific test for the identification of cardiac glycoside. Glycosides classification and isolation tests part 2. A brown ring formed between the layers which showed the entity of cardiac steroidal glycosides. Test for proteins various extracts were dissolved in few ml of water and treated with millons reagent. Take a piece of lamina or thick section of the leaf and add sodium picrate reagent. Test for glycosides keller kiliani test 5ml of extract was dissolved in 2 ml chloroform. Took extract solution in test tube and added few drops of fecl 3 in it.

Test for glycosides few gm of extract is diluted to 5 ml, with distilled water. The sugar moiety can be joined to the aglycone in various ways. Preliminary phytochemical screening of methanolic extract. Reagent used in the fluorescence test of coumarin glycoside. Borntragers, keller kiliani s tests and for the presence of cyanogenic glycosides. The antibacterial effect of some natural bioactive. Keller kiliani test and concentrate h 2 so 4 test confirmed the presence of glycosides in the methanolic plant extract. Cardiac glycosides are found in a diverse group of plants including digitalis purpurea and. Tests for tannins test for tannins was performed by. To 2 ml extract, glacial acetic acid, one drop 5% fecl 3 and conc. Reddish brown color appears at junction of the two liquid layers and upper layer. Purple ring with acid solution turning green presence of steroid 5. A brown ring at the interface indicates the presence of glycosides.